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Resource Source Identifier Antibodies Rabbit Polyclonal Anti E2f3 Antibody Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and morehttps://www.bioz.com/product/resource+source+identifier+antibodies+rabbit+polyclonal+anti+e2f3+antibody+proteintech/E2F3+Antibody/pm37734378-249-2-10
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Article Title: Signaling-induced systematic repression of miRNAs uncovers cancer vulnerabilities and targeted therapy sensitivity.
Journal: Cell reports. Medicine
doi: 10.1016/j.xcrm.2023.101200
Figure Legend Snippet: Figure 4. Drug prediction by miRNA sequencing uncovers drug sensitivities (A) Heatmap and unsupervised clustering according to the miRNA expression pattern of 12 included cancer models. (B) Overview of predicted activated pathways and corresponding PRSs for analyzed samples. The most frequent active pathways include WNT, TGF-b, and neurotophin (MAPK). (C) Overview of the top predicted target genes that are part of KEGG pathways. The most frequently predicted activated target genes are the cancer-associated genes E2F3 and CDK6.
Techniques Used: Sequencing, Expressing
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Recombinant:Article Title: Signaling-induced systematic repression of miRNAs uncovers cancer vulnerabilities and targeted therapy sensitivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal anti-E2F3 antibody Proteintech Cat# 27615-1-AP; RRID: AB_2880926 CDK6 (D4S8S) Rabbit mAb Cell Signaling Technology Cat# 13331; RRID: AB_2721897 Activin Receptor Type IIA antibody Biozol Cat# GTX107815; RRID: AB_2036157 Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb Cell Signaling Technology Cat# 3108; RRID: AB_490941 Smad2 (D43B4) XP Rabbit mAb Cell Signaling Technology Cat# 5339; RRID: AB_10626777 IGF-I Receptor b (D23H3) XP Rabbit mAb Cell Signaling Technology Cat# 9750; RRID: AB_10950969 Cyclin D2 (D52F9) Rabbit mAb Cell Signaling Technology Cat# 3741; RRID: AB_2070685 c-Cbl (C49H8) Rabbit mAb Cell Signaling Technology Cat# 2179; RRID: AB_823449 HER4/ErbB4 (111B2) Rabbit mAb Cell Signaling Technology Cat# 4795; RRID: AB_2099883 Rac1/2/3 Antibody Cell Signaling Technology Cat# 2465; RRID: AB_2176152 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat# 5174; RRID: AB_10622025 Mouse anti-alpha-Tubulin (clone B-5-1-2) Sigma Cat# T5168; RRID: AB_477579 HRP Rabbit anti-Mouse (polyclonal) Abcam Cat# ab6728; RRID: AB_955440 HRP Goat anti-Rabbit (polyclonal) Abcam Cat# ab6721; RRID: AB_955447 Biological samples Normal and tumor tissue from cancer patients from different entities University Hospital Heidelberg or Dresden N/A Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Thermo Fisher Scientific Cat# 12634028 Glucose Thermo Fisher Scientific Cat# 15023021 L-glutamine Thermo Fisher Scientific Cat# 25030024 HEPES Sigma-Aldrich Cat# H0887 Heparin Sigma-Aldrich Cat# H3149 BSA PAN-Biotech Cat# P06-10200 EGF, recombinant human R&D Systems Cat# 236-EG FGF basic, recombinant human R&D Systems Cat# 233-FB TGF beta 1, recombinant human Immunotools Cat# 11343160 Cultrex Reduced Growth Factor Basement Membrane Extract R&D Systems Cat# 3433-010-01 B-27TM Supplement Thermo Fisher Scientific Cat# A3582801 Penicillin-Streptomycin (10.000 U/ml) Thermo Fisher Scientific Cat# 15140148 Nicotinamide Sigma-Aldrich Cat# 72340-100G N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250-25G SB 202190 Sigma-Aldrich Cat# S7067-5MG A 83-01 Sigma-Aldrich Cat# SML0788-5MG Gastrin Sigma-Aldrich Cat# SCP0152-1MG Prostaglandin E2 Sigma-Aldrich Cat# P5640-1MG Primocin InvivoGen Cat# ant-pm-1 Y-27632 Stem Cell Technologies Cat# 72302 RPMI1640 with GlutaMax Thermo Fisher Scientific Cat# 61870036 FBS PAN-Biotech Cat# P40-47500 DMSO Sigma-Aldrich Cat# 472301 PBS Life Technologies Cat# 14190169 0.9% NaCl solution B.Braun Cat# 3570350 (Continued on next page) e1 Cell Reports Medicine 4, 101200, October 17, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Anisomycin Hoelzel Diagnostika Cat# HY-18982-10mM 5-Fluorouracil (5-FU) Hoelzel Diagnostika Cat# SIW-S0984 Bleomycin Hoelzel Diagnostika Cat# ACS-1994B Cisplatin Hoelzel Diagnostika Cat# A10221-10mM-D Ponatinib Hoelzel Diagnostika Cat# HY-12047-10mM Rapamycin Hoelzel Diagnostika Cat# A10782-10mM-D SB505124 Hoelzel Diagnostika Cat# A11157-5 Afatinib Biomol Cat# Cay11492-5 Lapatinib Biomol Cat# Cay11493-10 Linsitinib Biomol Cat# Cay17708-1 Palbociclib Biomol Cat# LKT-P014442.10 Dabrafenib Selleckchem Cat# S2807 Dacomitinib Selleckchem Cat# S2727 Pilaralisib Selleckchem Cat# S7645 Refametinib Selleckchem Cat# S1089 Trametinib Selleckchem Cat# S2673 Vemurafenib Selleckchem Cat# S1267 Cobimetinib Selleckchem Cat# S8041 GSK690693 MedChemExpress Cat# HY-10249 Ribociclib MedChemExpress Cat# HY-15777 Paclitaxel Biomol Cat# AG-CN2-0045-M025 SN38 Biozol Cat# SEL-S4908-10MG Oxaliplatin Biomol Cat# S1224 Leucovorin Selleckchem Cat# S1236 NaCl PanReac AppliChem Cat# A2942 Tris-HCL Carl Roth Cat# 9090-3 NP-40 Sigma-Aldrich Cat# 74385 Sodium deoxycholade Sigma-Aldrich Cat# 30970 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Cat# 05030 DTT PanReac AppliChem Cat# A2948 HiMarkTM Pre-stained Protein Standard ThermoFisher Cat# LC5699 Protease Inhibitor Cocktail Sigma-Aldrich Cat# S8830 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 PVDF membrane BioRad Cat# 162-0264 4x Laemmli Sample Buffer BioRad Cat# 1610747 Trans-Blot Turbo Transfer Buffer BioRad Cat# 10026938 Ponceau S Thermo Fisher Cat# A40000279 Tween 20 Fisher Scientific Cat# 10113103 Critical commercial assays NEXTFLEX Small RNA-Seq Kit v3 PerkinElmer Cat# NOVA-5132 miRNeasy RNA isolation mini kit Qiagen Cat# 217004 RNeasy FFPE Kit Qiagen Cat# 73504 SMARTer smRNA-Seq Kit for Illumina Takara Cat# 635031 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit Thermo Fisher Cat# 34580 XTT Cell Proliferation Assay Serva Cat# 39904.01 ATPlite 1step Luminescence Assay System Perkin Elmer Cat# 6016736 Bio Rad Protein Assay Dye Reagent concentrate BioRad Cat# 500-0006 (Continued on next page) Cell Reports Medicine 4, 101200, October 17, 2023 e2
Article Title: Signaling-induced systematic repression of miRNAs uncovers cancer vulnerabilities and targeted therapy sensitivity
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal anti-E2F3 antibody Proteintech Cat# 27615-1-AP; RRID: AB_2880926 CDK6 (D4S8S) Rabbit mAb Cell Signaling Technology Cat# 13331; RRID: AB_2721897 Activin Receptor Type IIA antibody Biozol Cat# GTX107815; RRID: AB_2036157 Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb Cell Signaling Technology Cat# 3108; RRID: AB_490941 Smad2 (D43B4) XP Rabbit mAb Cell Signaling Technology Cat# 5339; RRID: AB_10626777 IGF-I Receptor b (D23H3) XP Rabbit mAb Cell Signaling Technology Cat# 9750; RRID: AB_10950969 Cyclin D2 (D52F9) Rabbit mAb Cell Signaling Technology Cat# 3741; RRID: AB_2070685 c-Cbl (C49H8) Rabbit mAb Cell Signaling Technology Cat# 2179; RRID: AB_823449 HER4/ErbB4 (111B2) Rabbit mAb Cell Signaling Technology Cat# 4795; RRID: AB_2099883 Rac1/2/3 Antibody Cell Signaling Technology Cat# 2465; RRID: AB_2176152 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat# 5174; RRID: AB_10622025 Mouse anti-alpha-Tubulin (clone B-5-1-2) Sigma Cat# T5168; RRID: AB_477579 HRP Rabbit anti-Mouse (polyclonal) Abcam Cat# ab6728; RRID: AB_955440 HRP Goat anti-Rabbit (polyclonal) Abcam Cat# ab6721; RRID: AB_955447 Biological samples Normal and tumor tissue from cancer patients from different entities University Hospital Heidelberg or Dresden N/A Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Thermo Fisher Scientific Cat# 12634028 Glucose Thermo Fisher Scientific Cat# 15023021 L-glutamine Thermo Fisher Scientific Cat# 25030024 HEPES Sigma-Aldrich Cat# H0887 Heparin Sigma-Aldrich Cat# H3149 BSA PAN-Biotech Cat# P06-10200 EGF, recombinant human R&D Systems Cat# 236-EG FGF basic, recombinant human R&D Systems Cat# 233-FB TGF beta 1, recombinant human Immunotools Cat# 11343160 Cultrex Reduced Growth Factor Basement Membrane Extract R&D Systems Cat# 3433-010-01 B-27TM Supplement Thermo Fisher Scientific Cat# A3582801 Penicillin-Streptomycin (10.000 U/ml) Thermo Fisher Scientific Cat# 15140148 Nicotinamide Sigma-Aldrich Cat# 72340-100G N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250-25G SB 202190 Sigma-Aldrich Cat# S7067-5MG A 83-01 Sigma-Aldrich Cat# SML0788-5MG Gastrin Sigma-Aldrich Cat# SCP0152-1MG Prostaglandin E2 Sigma-Aldrich Cat# P5640-1MG Primocin InvivoGen Cat# ant-pm-1 Y-27632 Stem Cell Technologies Cat# 72302 RPMI1640 with GlutaMax Thermo Fisher Scientific Cat# 61870036 FBS PAN-Biotech Cat# P40-47500 DMSO Sigma-Aldrich Cat# 472301 PBS Life Technologies Cat# 14190169 0.9% NaCl solution B.Braun Cat# 3570350 (Continued on next page) e1 Cell Reports Medicine 4, 101200, October 17, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Anisomycin Hoelzel Diagnostika Cat# HY-18982-10mM 5-Fluorouracil (5-FU) Hoelzel Diagnostika Cat# SIW-S0984 Bleomycin Hoelzel Diagnostika Cat# ACS-1994B Cisplatin Hoelzel Diagnostika Cat# A10221-10mM-D Ponatinib Hoelzel Diagnostika Cat# HY-12047-10mM Rapamycin Hoelzel Diagnostika Cat# A10782-10mM-D SB505124 Hoelzel Diagnostika Cat# A11157-5 Afatinib Biomol Cat# Cay11492-5 Lapatinib Biomol Cat# Cay11493-10 Linsitinib Biomol Cat# Cay17708-1 Palbociclib Biomol Cat# LKT-P014442.10 Dabrafenib Selleckchem Cat# S2807 Dacomitinib Selleckchem Cat# S2727 Pilaralisib Selleckchem Cat# S7645 Refametinib Selleckchem Cat# S1089 Trametinib Selleckchem Cat# S2673 Vemurafenib Selleckchem Cat# S1267 Cobimetinib Selleckchem Cat# S8041 GSK690693 MedChemExpress Cat# HY-10249 Ribociclib MedChemExpress Cat# HY-15777 Paclitaxel Biomol Cat# AG-CN2-0045-M025 SN38 Biozol Cat# SEL-S4908-10MG Oxaliplatin Biomol Cat# S1224 Leucovorin Selleckchem Cat# S1236 NaCl PanReac AppliChem Cat# A2942 Tris-HCL Carl Roth Cat# 9090-3 NP-40 Sigma-Aldrich Cat# 74385 Sodium deoxycholade Sigma-Aldrich Cat# 30970 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Cat# 05030 DTT PanReac AppliChem Cat# A2948 HiMarkTM Pre-stained Protein Standard ThermoFisher Cat# LC5699 Protease Inhibitor Cocktail Sigma-Aldrich Cat# S8830 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 PVDF membrane BioRad Cat# 162-0264 4x Laemmli Sample Buffer BioRad Cat# 1610747 Trans-Blot Turbo Transfer Buffer BioRad Cat# 10026938 Ponceau S Thermo Fisher Cat# A40000279 Tween 20 Fisher Scientific Cat# 10113103 Critical commercial assays NEXTFLEX Small RNA-Seq Kit v3 PerkinElmer Cat# NOVA-5132 miRNeasy RNA isolation mini kit Qiagen Cat# 217004 RNeasy FFPE Kit Qiagen Cat# 73504 SMARTer smRNA-Seq Kit for Illumina Takara Cat# 635031 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit Thermo Fisher Cat# 34580 XTT Cell Proliferation Assay Serva Cat# 39904.01 ATPlite 1step Luminescence Assay System Perkin Elmer Cat# 6016736 Bio Rad Protein Assay Dye Reagent concentrate BioRad Cat# 500-0006 (Continued on next page) Cell Reports Medicine 4, 101200, October 17, 2023 e2 Article ll OPEN ACCESS
Membrane:Article Title: Signaling-induced systematic repression of miRNAs uncovers cancer vulnerabilities and targeted therapy sensitivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal anti-E2F3 antibody Proteintech Cat# 27615-1-AP; RRID: AB_2880926 CDK6 (D4S8S) Rabbit mAb Cell Signaling Technology Cat# 13331; RRID: AB_2721897 Activin Receptor Type IIA antibody Biozol Cat# GTX107815; RRID: AB_2036157 Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb Cell Signaling Technology Cat# 3108; RRID: AB_490941 Smad2 (D43B4) XP Rabbit mAb Cell Signaling Technology Cat# 5339; RRID: AB_10626777 IGF-I Receptor b (D23H3) XP Rabbit mAb Cell Signaling Technology Cat# 9750; RRID: AB_10950969 Cyclin D2 (D52F9) Rabbit mAb Cell Signaling Technology Cat# 3741; RRID: AB_2070685 c-Cbl (C49H8) Rabbit mAb Cell Signaling Technology Cat# 2179; RRID: AB_823449 HER4/ErbB4 (111B2) Rabbit mAb Cell Signaling Technology Cat# 4795; RRID: AB_2099883 Rac1/2/3 Antibody Cell Signaling Technology Cat# 2465; RRID: AB_2176152 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat# 5174; RRID: AB_10622025 Mouse anti-alpha-Tubulin (clone B-5-1-2) Sigma Cat# T5168; RRID: AB_477579 HRP Rabbit anti-Mouse (polyclonal) Abcam Cat# ab6728; RRID: AB_955440 HRP Goat anti-Rabbit (polyclonal) Abcam Cat# ab6721; RRID: AB_955447 Biological samples Normal and tumor tissue from cancer patients from different entities University Hospital Heidelberg or Dresden N/A Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Thermo Fisher Scientific Cat# 12634028 Glucose Thermo Fisher Scientific Cat# 15023021 L-glutamine Thermo Fisher Scientific Cat# 25030024 HEPES Sigma-Aldrich Cat# H0887 Heparin Sigma-Aldrich Cat# H3149 BSA PAN-Biotech Cat# P06-10200 EGF, recombinant human R&D Systems Cat# 236-EG FGF basic, recombinant human R&D Systems Cat# 233-FB TGF beta 1, recombinant human Immunotools Cat# 11343160 Cultrex Reduced Growth Factor Basement Membrane Extract R&D Systems Cat# 3433-010-01 B-27TM Supplement Thermo Fisher Scientific Cat# A3582801 Penicillin-Streptomycin (10.000 U/ml) Thermo Fisher Scientific Cat# 15140148 Nicotinamide Sigma-Aldrich Cat# 72340-100G N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250-25G SB 202190 Sigma-Aldrich Cat# S7067-5MG A 83-01 Sigma-Aldrich Cat# SML0788-5MG Gastrin Sigma-Aldrich Cat# SCP0152-1MG Prostaglandin E2 Sigma-Aldrich Cat# P5640-1MG Primocin InvivoGen Cat# ant-pm-1 Y-27632 Stem Cell Technologies Cat# 72302 RPMI1640 with GlutaMax Thermo Fisher Scientific Cat# 61870036 FBS PAN-Biotech Cat# P40-47500 DMSO Sigma-Aldrich Cat# 472301 PBS Life Technologies Cat# 14190169 0.9% NaCl solution B.Braun Cat# 3570350 (Continued on next page) e1 Cell Reports Medicine 4, 101200, October 17, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Anisomycin Hoelzel Diagnostika Cat# HY-18982-10mM 5-Fluorouracil (5-FU) Hoelzel Diagnostika Cat# SIW-S0984 Bleomycin Hoelzel Diagnostika Cat# ACS-1994B Cisplatin Hoelzel Diagnostika Cat# A10221-10mM-D Ponatinib Hoelzel Diagnostika Cat# HY-12047-10mM Rapamycin Hoelzel Diagnostika Cat# A10782-10mM-D SB505124 Hoelzel Diagnostika Cat# A11157-5 Afatinib Biomol Cat# Cay11492-5 Lapatinib Biomol Cat# Cay11493-10 Linsitinib Biomol Cat# Cay17708-1 Palbociclib Biomol Cat# LKT-P014442.10 Dabrafenib Selleckchem Cat# S2807 Dacomitinib Selleckchem Cat# S2727 Pilaralisib Selleckchem Cat# S7645 Refametinib Selleckchem Cat# S1089 Trametinib Selleckchem Cat# S2673 Vemurafenib Selleckchem Cat# S1267 Cobimetinib Selleckchem Cat# S8041 GSK690693 MedChemExpress Cat# HY-10249 Ribociclib MedChemExpress Cat# HY-15777 Paclitaxel Biomol Cat# AG-CN2-0045-M025 SN38 Biozol Cat# SEL-S4908-10MG Oxaliplatin Biomol Cat# S1224 Leucovorin Selleckchem Cat# S1236 NaCl PanReac AppliChem Cat# A2942 Tris-HCL Carl Roth Cat# 9090-3 NP-40 Sigma-Aldrich Cat# 74385 Sodium deoxycholade Sigma-Aldrich Cat# 30970 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Cat# 05030 DTT PanReac AppliChem Cat# A2948 HiMarkTM Pre-stained Protein Standard ThermoFisher Cat# LC5699 Protease Inhibitor Cocktail Sigma-Aldrich Cat# S8830 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 PVDF membrane BioRad Cat# 162-0264 4x Laemmli Sample Buffer BioRad Cat# 1610747 Trans-Blot Turbo Transfer Buffer BioRad Cat# 10026938 Ponceau S Thermo Fisher Cat# A40000279 Tween 20 Fisher Scientific Cat# 10113103 Critical commercial assays NEXTFLEX Small RNA-Seq Kit v3 PerkinElmer Cat# NOVA-5132 miRNeasy RNA isolation mini kit Qiagen Cat# 217004 RNeasy FFPE Kit Qiagen Cat# 73504 SMARTer smRNA-Seq Kit for Illumina Takara Cat# 635031 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit Thermo Fisher Cat# 34580 XTT Cell Proliferation Assay Serva Cat# 39904.01 ATPlite 1step Luminescence Assay System Perkin Elmer Cat# 6016736 Bio Rad Protein Assay Dye Reagent concentrate BioRad Cat# 500-0006 (Continued on next page) Cell Reports Medicine 4, 101200, October 17, 2023 e2
Article Title: Signaling-induced systematic repression of miRNAs uncovers cancer vulnerabilities and targeted therapy sensitivity
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal anti-E2F3 antibody Proteintech Cat# 27615-1-AP; RRID: AB_2880926 CDK6 (D4S8S) Rabbit mAb Cell Signaling Technology Cat# 13331; RRID: AB_2721897 Activin Receptor Type IIA antibody Biozol Cat# GTX107815; RRID: AB_2036157 Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb Cell Signaling Technology Cat# 3108; RRID: AB_490941 Smad2 (D43B4) XP Rabbit mAb Cell Signaling Technology Cat# 5339; RRID: AB_10626777 IGF-I Receptor b (D23H3) XP Rabbit mAb Cell Signaling Technology Cat# 9750; RRID: AB_10950969 Cyclin D2 (D52F9) Rabbit mAb Cell Signaling Technology Cat# 3741; RRID: AB_2070685 c-Cbl (C49H8) Rabbit mAb Cell Signaling Technology Cat# 2179; RRID: AB_823449 HER4/ErbB4 (111B2) Rabbit mAb Cell Signaling Technology Cat# 4795; RRID: AB_2099883 Rac1/2/3 Antibody Cell Signaling Technology Cat# 2465; RRID: AB_2176152 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat# 5174; RRID: AB_10622025 Mouse anti-alpha-Tubulin (clone B-5-1-2) Sigma Cat# T5168; RRID: AB_477579 HRP Rabbit anti-Mouse (polyclonal) Abcam Cat# ab6728; RRID: AB_955440 HRP Goat anti-Rabbit (polyclonal) Abcam Cat# ab6721; RRID: AB_955447 Biological samples Normal and tumor tissue from cancer patients from different entities University Hospital Heidelberg or Dresden N/A Chemicals, peptides, and recombinant proteins Advanced DMEM/F-12 Thermo Fisher Scientific Cat# 12634028 Glucose Thermo Fisher Scientific Cat# 15023021 L-glutamine Thermo Fisher Scientific Cat# 25030024 HEPES Sigma-Aldrich Cat# H0887 Heparin Sigma-Aldrich Cat# H3149 BSA PAN-Biotech Cat# P06-10200 EGF, recombinant human R&D Systems Cat# 236-EG FGF basic, recombinant human R&D Systems Cat# 233-FB TGF beta 1, recombinant human Immunotools Cat# 11343160 Cultrex Reduced Growth Factor Basement Membrane Extract R&D Systems Cat# 3433-010-01 B-27TM Supplement Thermo Fisher Scientific Cat# A3582801 Penicillin-Streptomycin (10.000 U/ml) Thermo Fisher Scientific Cat# 15140148 Nicotinamide Sigma-Aldrich Cat# 72340-100G N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250-25G SB 202190 Sigma-Aldrich Cat# S7067-5MG A 83-01 Sigma-Aldrich Cat# SML0788-5MG Gastrin Sigma-Aldrich Cat# SCP0152-1MG Prostaglandin E2 Sigma-Aldrich Cat# P5640-1MG Primocin InvivoGen Cat# ant-pm-1 Y-27632 Stem Cell Technologies Cat# 72302 RPMI1640 with GlutaMax Thermo Fisher Scientific Cat# 61870036 FBS PAN-Biotech Cat# P40-47500 DMSO Sigma-Aldrich Cat# 472301 PBS Life Technologies Cat# 14190169 0.9% NaCl solution B.Braun Cat# 3570350 (Continued on next page) e1 Cell Reports Medicine 4, 101200, October 17, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Anisomycin Hoelzel Diagnostika Cat# HY-18982-10mM 5-Fluorouracil (5-FU) Hoelzel Diagnostika Cat# SIW-S0984 Bleomycin Hoelzel Diagnostika Cat# ACS-1994B Cisplatin Hoelzel Diagnostika Cat# A10221-10mM-D Ponatinib Hoelzel Diagnostika Cat# HY-12047-10mM Rapamycin Hoelzel Diagnostika Cat# A10782-10mM-D SB505124 Hoelzel Diagnostika Cat# A11157-5 Afatinib Biomol Cat# Cay11492-5 Lapatinib Biomol Cat# Cay11493-10 Linsitinib Biomol Cat# Cay17708-1 Palbociclib Biomol Cat# LKT-P014442.10 Dabrafenib Selleckchem Cat# S2807 Dacomitinib Selleckchem Cat# S2727 Pilaralisib Selleckchem Cat# S7645 Refametinib Selleckchem Cat# S1089 Trametinib Selleckchem Cat# S2673 Vemurafenib Selleckchem Cat# S1267 Cobimetinib Selleckchem Cat# S8041 GSK690693 MedChemExpress Cat# HY-10249 Ribociclib MedChemExpress Cat# HY-15777 Paclitaxel Biomol Cat# AG-CN2-0045-M025 SN38 Biozol Cat# SEL-S4908-10MG Oxaliplatin Biomol Cat# S1224 Leucovorin Selleckchem Cat# S1236 NaCl PanReac AppliChem Cat# A2942 Tris-HCL Carl Roth Cat# 9090-3 NP-40 Sigma-Aldrich Cat# 74385 Sodium deoxycholade Sigma-Aldrich Cat# 30970 Sodium dodecyl sulfate (SDS) Sigma-Aldrich Cat# 05030 DTT PanReac AppliChem Cat# A2948 HiMarkTM Pre-stained Protein Standard ThermoFisher Cat# LC5699 Protease Inhibitor Cocktail Sigma-Aldrich Cat# S8830 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat# P0044 PVDF membrane BioRad Cat# 162-0264 4x Laemmli Sample Buffer BioRad Cat# 1610747 Trans-Blot Turbo Transfer Buffer BioRad Cat# 10026938 Ponceau S Thermo Fisher Cat# A40000279 Tween 20 Fisher Scientific Cat# 10113103 Critical commercial assays NEXTFLEX Small RNA-Seq Kit v3 PerkinElmer Cat# NOVA-5132 miRNeasy RNA isolation mini kit Qiagen Cat# 217004 RNeasy FFPE Kit Qiagen Cat# 73504 SMARTer smRNA-Seq Kit for Illumina Takara Cat# 635031 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit Thermo Fisher Cat# 34580 XTT Cell Proliferation Assay Serva Cat# 39904.01 ATPlite 1step Luminescence Assay System Perkin Elmer Cat# 6016736 Bio Rad Protein Assay Dye Reagent concentrate BioRad Cat# 500-0006 (Continued on next page) Cell Reports Medicine 4, 101200, October 17, 2023 e2 Article ll OPEN ACCESS
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